ds_cbreaker_ds_1s4_cif
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| - | Chromosome breakage Loss(due to Ds) of Clf1 in heterozygous kernel (loss of purple aleurone) and in F1 leaf; defective albino phenotype in sectors; proximal pattern. | ||
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| - | colorless floury defective; colorless kernel sector: | ||
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| - | A selfed ear of Ac Dek1 Ds-1S4/- dek1 plant showing 3 to 1 segregation for intensely purple kernels, including many with large colorless patches and lacy-like colored areas, and for colorless floury partially collapsed dek1 kernels. The frequency of the colorless dek1 kernels appears to be reduced, but this is only the consequence of the fact that some of the colorless floury kernels are collapsed and defective and are not always seen on the photograph. | ||
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| - | Enlargement of kernels from the selfed ear showing Ac Dek Ds/- dek/dek (middle 2 kernels in top row) and Ac Dek Ds/Ac Dek Ds/dek (dark kernel in lower row). The middle two kernels in the top row show large colorless patches with chains of dots and isolated islands of intensely colored tissue. The bottom dark kernel shows mostly colored tissue interspersed with small lighter patches from overlapping events with a double dose of Dek Ds-1S4. | ||
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| - | Kernel enlargement. Note that some of the colored areas have succeeding small colorless spots (loss of Dek) and some do not | ||
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| - | Kernels from an Ac Dek Ds/dek/dek ear showing good examples of the behavior of acentric fragments in the cell lineages of the aleurone. | ||
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| - | Enlargement of the far right-hand kernel in ear below. The kernel is Ac Dek Ds/dek/dek showing good examples of the behavior of acentric fragments in the cell lineages of the aleurone. Note the islands and chains of recovery spots, including one example (lower left) where the surviving fragment was carried along and maintained very well, producing a large thumb-like area of intensely pigmented tissue. Nearby are several small islands that represent lineages or replication of the acentric fragments that did not keep up with cell division, thus producing lineages from daughter cells that did not always retain a functional Dek1. To the right are patches of colored tissue with colorless loss areas within their borders, suggestive of fully functional lineages with periodic losses because an active Ds and Dek1 gene were on a chromosome arm still attached to the centromere. | ||
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| - | Section of the top surface of the 8th leaf of a normal sib (left) and the Ac Dek Ds-1S4/- dek original plant (right) showing the consequences of the Ac Ds induced losses in heterozygous leaf tissue. No white streaks are apparent on the top, but the cell lineages are defective and did not survive well, producing the rough appearance on the top of the leaf. This is because the surviving cells grow into the empty spaces left by the dead cells. This is a unique characteristic of Ds-1S4 as opposed to the other Ds-1S mutants. It is not known why the cells did not survive. Normally albino cells survive because surrounding cells will feed them and keep them alive. Perhaps some other gene is being lost besides Dek1. The segment lost must have had something vital to the development of leaf tissue. The Ds leaf has a wrinkled bumpy surface, darker in color, less reflective surface and irregular elongated indentations, | ||
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| - | Section of the underneath surfaces of the leaves described in image 8 above, showing the 8th leaf of a normal sib (left) and the Ac Dek Ds-1S4/- dek original plant (right). The ladder-like appearance of the vein network on the undersurface of the leaf is an effect produced by the wrinkles protruding on the underneath where they are indented on the top surface. | ||
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ds_cbreaker_ds_1s4_cif.1394720180.txt.gz · Last modified: 2014/03/13 14:16 by chris.bottoms