Ds-Cbreaker O5; (O5 Ds-7L1) A selfed ear with all colored kernels from the original Ac/- O5 Ds-7L1/o5 plant showing semisterile seed set (due to failure of homozygous o5 kernels, which can be extremely collapsed, to become established on the ear) and segregation for purple kernels versus pitted etched mosaic kernels (probably Cp Ds/cp/cp) indicating frequent late loss of Cp in the endosperm due to Ds breakage on the distal side of the O5 locus. Most of the combinations of the 3N endosperm that have more than one dominant Cp allele or more than one Ac or no Ac at all will have no visible cp sectors because of the characteristic late occurrence of the breaking event in this case. The presence of Ac is confirmed by the red P-v streaks on the pericarp of many of the kernels.

Ds-Cbreaker O5; (O5 Ds-7L1) Top leaf surface of an Ac/-, Cp Ds-7L1/cp plant showing many small elongated lighter green and whitish sectors ranging from pencil-lead sized down to a very few cells. Some of the larger ones are almost white, like the cp cp seedling phenotype.

Ds-Cbreaker O5; (O5 Ds-7L1) Underside surface of the same leaf as Image 3 above, showing the same phenotype except that the nearly white expression of the longer sectors is more pronounced. Also there is some indication of latent necrosis of the larger sector which is characteristic of the allele cp*-76 used in these experiments. Note that none of these sectors have an effect on the structure of the leaf as seen in Ds-1S4.